• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
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    • 专利摘要:
    1. A method for producing a glucose-freezing syrup by contacting a glucose solution with a catalyst, which is a glucose isomerase, adsorbed on silicon dioxide, characterized in that, in order to increase the productivity of the process and increase the service life of the catalyst, the initial solution is precontacted with briquette from a sugar binder, and they will be bled with a binder from a lid and blued material, and the service life of the catalysts will increase the service life. or aluminosilicate when the mass ratio of the catalyst and the specified briquettes 1:
    公开号:SU1194286A3
    申请号:SU823526949
    申请日:1982-11-09
    公开日:1985-11-23
    发明作者:Вейденбах Гюнтер；Бонзе Дирк；Мейер Борис
    申请人:Кали-Хеми Аг (Фирма)；
    IPC主号:
    专利说明:

    "
    The invention relates to a method for preparing a solution containing glucose and fructose by converting a glucose solution on a catalyst which is based on a SiOj carrier. and possesses glucose isomerase activity. Recently, the enzymatic conversion of glucose into a glucose fructose mixture has become increasingly important. At the same time, such a mixture is mainly implemented in the form of a syrup, called isomer, and is primarily intended to be replaced in the food and non-alcoholic industries. expensive and all the less common in the world of crystalline sugar (cane sugar, beet sugar, sucrose), obtained from strasnit or sugar beet. Natural starches, such as corn and potato starches, are converted into glucose by acidic or enzymatic hydrolysis as a source of glucose, which is necessary for the preparation of a glucose-fusion mixture.
    For the enzymatic conversion of glucose to fructose, an aqueous solution of glucose is used, if necessary with the addition of isomerization agents (for example, cobalt or magnesium ions), upon exposure to which a given degree of isomerization can be achieved, after which the additives added are separated from the solution and the solution cry until syrup consistency.
    The aim of the invention is to increase the productivity of the process and increase the service life of the catalyst.
    The method of preparation of the proposed solution containing glucose and fructose is based on the fact that the glucose solution (substrate) is passed through a reactor in which there is a carrier catalyst with glucose isomerase activity, and the initial solution is precontacted in contact with silica or aluminosilicate briquettes at a mass ratio catalyst and these briquettes 1: (1-3), mainly 1: 1.
    The competitiveness of isomerase in relation to natural sucrose
    62
    together with the price, it is mainly determined by the content of fructose, as this is a measure of sweetness. As opposed to natural
    sucrose, glucose disaccharose and fructose, in which the molecular ratio of glucose and fructose is 1: 1, the content of glucose and fructose in isomerase and, consequently, its sweetness
    fluctuates and depends on the time of action of the glucose isomerase on the glucose solution and on the temperature at which this effect takes place. Thermodynamic equilibrium, which
    maximum is reached at 60 ° C after a rather long time, it takes place with an isomerization degree of 51%, i.e Per 100 glucose molecules introduced, there are 51 fructose molecules. Known isomerase content contains 42% fructose in a dry substance. Since the glucose content of the technical starting product is 90-95%, in practice it is necessary to have a degree
    isomerization of 44-47% to achieve the indicated fructose concentration. To achieve this degree of isomerization, supported catalysts with glucose isomerase activity can
    have only a certain volumetric rate corresponding to their activity. Modern highly active catalysts can move with an initial bulk velocity from 10
    BEFORE ,
    With increasing processing time, the activity of known catalysts decreases more or less rapidly, depending on the reaction temperature.
    The duration of the operation of the catalysts with glucose isomerase activity based on SiO can be more than doubled if the glucose solution before the reaction to
    catalyst carrier is in contact
    with briquettes SiO, or aluminosilicate. The mass ratio of catalyst and briquettes varies in the range of 3: 1 1: 3, preferably 1: 1.
    With this method, the performance
    catalyst is doubled. Productivity is determined by the amount of substrate, calculated on the dry substance (kg), which at
    A given degree of isomerization may be treated with 1 kg of KaTajui3aTopa to its residual activity equal to 20% of the initial activity. 3 in a device for the continuous process of converting glucose to fructose, in which, along with a reactor for a carrier catalyst with glucose isomerase activity, there are also necessary elements such as storage and receiving tanks, pipelines, pumps, gauges, temperature controllers before the reactor has a SiOji, or aluminosilicate briquette column. At the same time, the volume of the column in relation to the volume of the reactor must be such that, taking into account the bulk weights of the catalyst and the material filling the column, the ratio of their masses will have the indicated values. The proposed continuous method is carried out in such a way that the water solution of glucose, in which the dry matter content is preferably 40-50% and having a pH of 7-8.5, the solution is heated to 55-60 ° C and after this is pumped through SiOj pretreatment column. or with aluminosilicate and then through a reactor filled with a catalyst catalyst based on SiOt with glucose isomerase activity. The flow of glucose and fructose at certain intervals is analyzed for the content of fructose, for example, by means of polarimetric analysis and. The volumetric rate of the catalyst is set such that the flow of fructose in the flowing out plant is 42 wt.% Of dry matter. Most preferably, the particle size of the material in the pretreatment column is 0.5-5.0 mm and the particle size of the carrier catalyst is 0.080, 5 mm. If Streptomyces Albus uses glucose isomerase to prepare a catalyst carrier with glucose isomerase activity, it is most advisable to have a solution pH of 7.0-8.5 and heat this solution to 55-65 Moreover, when using Streptomyces Albus glucose isomerase To accelerate isomeric pi, it is advantageous to add Co (II) and Mg (II) ions to a solution of glucose, preferably in the form of their aqueous solutions, such as chlorides or sulfates, in an amount of 0.1-2 ppmCo (P) and in the amount of 10-200 ppm Mg (II). It is also preferred to add a stabilizing amount of an anti-oxidant, preferably ZrO, to the glucose solution. in the amount of 100-600 ppm in the form of sulfites or alkali metal bisulfites. Before concentrating the glucose and fructose solution into the syrup or until the final application of the solution, it is recommended to remove undesirable ionic components from it, such as impairing the taste, by means of anion exchangers and cation exchangers. Example 1. 5 g of a catalyst with glucose isomerase activity is introduced into the reactor in front of which there is a column in which there are 5 g of commercial, waterproof porous, spherical aluminosilicate (for example, type KCT-WS Kali-Chemie Ag, containing 97% by weight SiOj And 3 wt.% Oz). Through this system, a glucose solution heated to 60 ° C is pumped. The volumetric rate (related to the reactor volume occupied by the catalyst) is set such that the degree of isomerization during the whole period of operation is constantly 46.5%. The degree of isomerization of the resulting substrate solution is measured by the polarization method. The catalyst, the preliminary stage filler (columns) and the method are characterized by the following parameters: 1. Catalyst 1.1 carrier SiOi 1.2 activity, U / r 9000 1.3 grain, 0.1-0.2 1.4 1.4 bulk (dry) 0.45 kg / 1 2. The filler of the column 2.1 material KCT-WS (Kali-Chemie Ag) 2.2 grain size, mm1-2 2.3 bulk weight (dry), kg / 1 0.70 3. Method 3.1substrat45 wt% glucose in an aqueous solution 3.2.220 ppm Md (U) 1 ppm CO, (a) 200 ppm SOi8 (B NajSO,) 3.3pH7.5 3.4 substrate density, kg / 1 1.2 3.5 substrate temperature at the entrance, C60 3.6 isomerization degree,% 46.5 3.7 initial volumetric rate, h 13.0 4. Def. Leniye glucose isomerase activity sol solution glyukozoi Meraz activity intended for direct bev- erages catalyst is determined by the method of Takasaki. The unit of activity (s) is determined by the amount of enzyme with which it forms 1 mg of fructose under incubation conditions. . Incubation conditions: temperature, C 1 reaction time, h 0.1 gluco substrate Hj, 0 (Mer 8342) in 0 phosphate buffer, pH 0.0004 t M The following results are obtained: half-life, h 1300 operating time to a residual activity of 20%, h average activity 44 (over from after,% 3800 hours of work to start active 100%) 26,000 kg TS productivity with 46.5% fruit after 3800 hours of operation) kg of catalyst Example .2. 5 g of the catalyst catalyst of Example 1 were introduced into the reactor, in front of which there is a column containing 10 g of commercial spherical, waterproof, porous SiO, i (for example, type AF 125 der. Kali-Chemie Ag, in which the SiO content is greater than 99%, %), The method was carried out: as in Example 1. The filler of the column had the following characteristics: AF 125 (Kali1. Chemie Ag material) 2. Grain size, mm 3. Powder weight (dry), kg / l 0.45 The results were identical to those of example 1, Example 3. Comparison of the experiment was carried out with a catalyst corresponding to example 1, however without prior processing steps in the column, and all other things being equal. The following results are obtained: time, half-life, h 670, work time until reaching 20%. residual activity, h 47.2 (relative activity after 1700 h,% of initial activity) 12.500 kg TS productivity after 1700 h with 46.5% fructose / kg catalyst.
    权利要求:
    Claims (2)
    [1]
    1. METHOD FOR PRODUCING Glucose Fructose Syrup by contacting a glucose solution with a catalyst that is a glucose isomerase adsorbed onto silicon dioxide, characterized in that, in order to increase the productivity of the process and increase the service life of the catalyst, the initial solution is preliminarily contacted with briquettes of silicon dioxide or aluminosilicate with a ratio of the mass of the catalyst and these briquettes 1: (1-3).
    [2]
    2. The method according to claim 1, characterized in that the process is carried out with a mass ratio of the catalyst and briquettes of silicon dioxide or aluminosilicate 1: 1.
    S go
    QO smiling
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    同族专利:
    公开号 | 公开日
    HU191480B|1987-02-27|
    DE3260637D1|1984-10-04|
    YU270782A|1985-03-20|
    DE3148603C1|1983-07-21|
    AU9151982A|1983-06-16|
    AU559865B2|1987-03-19|
    DD205697A5|1984-01-04|
    RO87007A|1985-06-29|
    ES517978A0|1983-09-01|
    DK544582A|1983-06-10|
    AT9167T|1984-09-15|
    DK158363C|1990-10-15|
    PL141192B1|1987-07-31|
    US4533633A|1985-08-06|
    AR228218A1|1983-01-31|
    ES517977A0|1983-09-01|
    ES8308587A1|1983-09-01|
    EP0081185A1|1983-06-15|
    CS239934B2|1986-01-16|
    FI824204A0|1982-12-07|
    YU42612B|1988-10-31|
    FI72142B|1986-12-31|
    FI824204L|1983-06-10|
    DK158363B|1990-05-07|
    RO87007B|1985-06-30|
    ES8308588A1|1983-09-01|
    MX7436E|1988-11-09|
    EP0081185B1|1984-08-29|
    CA1194826A|1985-10-08|
    PL239403A1|1983-08-01|
    KR870001933B1|1987-10-22|
    FI72142C|1987-04-13|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    JPS5649118B2|1974-10-04|1981-11-19|
    JPS5515200B2|1975-02-21|1980-04-22|
    US4040861A|1976-03-15|1977-08-09|Cpc International Inc.|Process of refining enzymatically produced levulose syrups|
    IL51672D0|1976-04-05|1977-05-31|Miles Lab|Process and apparatus for carrying out enzymatic reactions in serially connected reactors|
    US4373025A|1976-05-27|1983-02-08|Uop Inc.|Process for the isomerization of glucose|
    DE2726188C2|1977-06-10|1979-05-10|Kali-Chemie Ag, 3000 Hannover|Process for the production of a water-insoluble enzyme preparation|
    US4226639A|1979-05-25|1980-10-07|Uop Inc.|Silica guard bed for adsorbent used in an aqueous system|
    US4288548A|1979-11-15|1981-09-08|Standard Brands Incorporated|Process for isomerizing glucose to fructose|DE3405035C1|1984-02-13|1985-04-25|Kali-Chemie Ag, 3000 Hannover|Process for the production of isoglucose|
    DE3719324C1|1987-06-10|1988-12-15|Kali Chemie Ag|Process for the production of carrier-bound enzymes|
    US5952205A|1998-02-06|1999-09-14|Neose Technologies, Inc.|Process for processing sucrose into glucose and fructose|
    US5998177A|1998-11-19|1999-12-07|Neose Technologies, Inc.|Process for processing sucrose into glucose|
    EA017266B1|2009-06-17|2012-11-30|Институт Катализа Им. Г.К. Борескова Сибирского Отделения Российской Академии Наук|Biocatalyst, method of its preparation and method of obtaining glucose-fructose syrups|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE3148603A|DE3148603C1|1981-12-09|1981-12-09|Process and plant for the production of isomerose|
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